Fig. 3. Deletion of miRNA-22 induces cardiac hypertrophy in female mice. (A) miR-22 expression levels in hearts of WT female mice fed a chow diet or obesogenic diet analyzed by qPCR (n=5-9). (B) Heart weight normalized by tibia length (HW/TL) (n=7-11). (C) Analysis of cardiomyocyte area of heart transverse sections using wheat germ agglutinin staining and (D) quantification of cardiomyocyte area evaluated by ImageJ; bars 50µm (n=3-4). (E) Analysis of gene expression of hypertrophic markers and collagen evaluated by qPCR in the heart of the mice (n=3-5). (F) Analysis of fibrotic area of heart transverse sections using Picrosirius Red staining and (G) quantification of fibrosis area evaluated by ImageJ; bars 100µm (n=2-4). * WT obesogenic diet vs WT chow diet (p<0.05); # KO obesogenic diet vs KO chow diet (p<0.05); & KO obesogenic diet vs WT obesogenic diet (p<0.05); § KO chow diet vs WT chow diet (p<0.05); Δ KO obesogenic diet vs WT chow diet (p<0.05).